8DMQ

Crystal structure of Legionella pneumophila macrodomain MavL in complex with ubiquitin vinyl methyl ester

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 31 2 1
Unit cell lengths	108.719, 108.719, 209.143
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	69.710 - 2.195
R-factor	0.1792
Rwork	0.178
R-free	0.20950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6omi 1ubq
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.710	2.273
High resolution limit [Å]	2.195	2.195
Number of reflections	73681	7171
<I/σ(I)>	14.95
Completeness [%]	99.7
Redundancy	10.3
CC(1/2)	0.978	0.725

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M sodium citrate, 20% w/v PEG 3350