8DKD

Sliding clamp from M. thermoresistibile

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2019-03-06
Detector	RDI CMOS_8M
Wavelength(s)	1
Spacegroup name	P 31 2 1
Unit cell lengths	102.408, 102.408, 134.159
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.970 - 3.200
R-factor	0.2593
Rwork	0.257
R-free	0.27710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3p16
RMSD bond length	0.002
RMSD bond angle	0.506
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.790	3.200
High resolution limit [Å]	3.200	3.200
Rmerge	0.084	0.910
Rpim	0.030
Number of reflections	12983	243
<I/σ(I)>	24.8
Completeness [%]	93.0
Redundancy	8.2
CC(1/2)	0.990	0.595

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	4 M ammonium acetate , 0.1 M bis-tris propane, 8% glycerol