8DJQ

Sliding-clamp-DnaE1 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	66.810, 148.252, 81.952
Unit cell angles	90.00, 92.18, 90.00

Refinement procedure

Resolution	19.870 - 2.800
R-factor	0.2281
Rwork	0.226
R-free	0.28490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8dj6
RMSD bond length	0.002
RMSD bond angle	0.479
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.062	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.153
Rpim	0.098
Number of reflections	38932	1673
<I/σ(I)>	4.75
Completeness [%]	99.5
Redundancy	3.3
CC(1/2)	0.967	0.967

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.5M Ammonium sulphate, 0.1 M sodium acetate tribasic pH 5.6