8DIR

The complex structure between human IgG1 Fc and its high affinity receptor FcgRI H174R variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2019-06-03
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	204.286, 89.276, 56.032
Unit cell angles	90.00, 97.72, 90.00

Refinement procedure

Resolution	36.870 - 2.300
R-factor	0.198
Rwork	0.196
R-free	0.23280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4x4m
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.070	0.464
Number of reflections	44290	2235
<I/σ(I)>	25.3	2.6
Completeness [%]	99.8
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% PEG3350, 0.2M Sodium Formate (pH6.6)