8DIO

Crystal structure of LARP1-DM15 from Danio rerio bound to m7GpppC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97931
Spacegroup name	P 1 21 1
Unit cell lengths	47.950, 59.702, 60.381
Unit cell angles	90.00, 100.31, 90.00

Refinement procedure

Resolution	47.180 - 2.300
R-factor	0.2123
Rwork	0.207
R-free	0.26010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5v87
RMSD bond length	0.012
RMSD bond angle	1.292
Data reduction software	AutoProcess
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.180	2.382
High resolution limit [Å]	2.300	2.300
Rmerge	0.015
Number of reflections	13814	1391
<I/σ(I)>	28.43
Completeness [%]	91.8	93.8
Redundancy	2	2
CC(1/2)	0.999	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	50 mM HEPES, pH 7, 0 or 100 mM NaCl, 2 mM DTT