8DHU

Crystal structure of LARP-DM15 from Drosophila melanogaster bound to m7GpppC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97931
Spacegroup name	P 21 21 21
Unit cell lengths	46.808, 60.737, 129.199
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.010 - 2.295
Rwork	0.220
R-free	0.27490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5v87
RMSD bond length	0.008
RMSD bond angle	1.221
Data reduction software	AutoProcess
Data scaling software	AutoProcess
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.010	2.377
High resolution limit [Å]	2.295	2.295
Rmerge	0.020	0.263
Rmeas	0.028	0.372
Number of reflections	17090	1695
<I/σ(I)>	17.01	2.31
Completeness [%]	99.7
Redundancy	2
CC(1/2)	1.000	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295.15	0.05 M Bicine, pH 9, 2.5% PEG 6000