8DHR

An ester mutant of SfGFP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	129.917, 37.492, 91.848
Unit cell angles	90.00, 106.31, 90.00

Refinement procedure

Resolution	44.080 - 1.750
R-factor	0.1808
Rwork	0.181
R-free	0.18830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.150	1.780
High resolution limit [Å]	1.750	1.750
Number of reflections	42753	2475
<I/σ(I)>	15.5	2.4
Completeness [%]	99.3
Redundancy	3.5
CC(1/2)	0.990	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	50 mM Hepes, pH 7.5 and 25% Peg4000