8DGR
Crystal Structure of scFv(F8) Antibody Fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979340 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 119.644, 119.644, 74.856 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.530 - 1.560 |
| Rwork | 0.175 |
| R-free | 0.19490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5b3n |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.736 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0350) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.530 | 1.600 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Number of reflections | 367580 | 2582 |
| <I/σ(I)> | 10708.8 | |
| Completeness [%] | 99.6 | 95.52 |
| Redundancy | 19.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 1.8 M ammonium sulfate, 0.2 M magnesium chloride, 0.1 M HEPES, 5 % glycerol |
