8DGD

Crystal Structure of GAF domain-containing protein, from Klebsiella pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2021-07-15
Detector	RAYONIX MX-300
Wavelength(s)	0.97856
Spacegroup name	C 2 2 21
Unit cell lengths	70.680, 114.380, 73.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.640 - 1.900
R-factor	0.1763
Rwork	0.172
R-free	0.21960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vhm
RMSD bond angle	13.942
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.950
High resolution limit [Å]	1.900	1.900
Rmerge	0.059	0.556
Number of reflections	23992	1754
<I/σ(I)>	23.49	3.92
Completeness [%]	100.0
Redundancy	6.92
CC(1/2)	0.990	0.902

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	290	Morpheus B3: 10% w/v PEG 4000, 20% v/v glycerol, 0.03M of each halide (0.3M sodium fluoride, 0.3M sodium bromide, 0.3M sodium iodide),0.1M MES/imidazole pH6.5: 321508B3, cok0-10