8DFX
Crystal structure of Human BTN2A1-BTN3A1 Ectodomain Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 114.630, 138.930, 336.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.350 - 5.550 |
| R-factor | 0.2942 |
| Rwork | 0.290 |
| R-free | 0.33610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f80 BTN2A1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.910 |
| Data reduction software | MOSFLM |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4 (3-FEB-2022)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.400 | 6.620 |
| High resolution limit [Å] | 5.550 | 5.550 |
| Rpim | 0.086 | 0.558 |
| Number of reflections | 2500 | 278 |
| <I/σ(I)> | 5.3 | 1.6 |
| Completeness [%] | 85.9 | 49.2 |
| Redundancy | 7.4 | 7.6 |
| CC(1/2) | 0.919 | 0.373 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 6% PEG 6000, 0.1 M magnesium sulfate, 0.1 M HEPES |
