8DFW

Crystal Structure of Human BTN2A1 in Complex With Vgamma9-Vdelta2 T Cell Receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	C 2 2 21
Unit cell lengths	112.030, 218.460, 107.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.720 - 2.100
R-factor	0.2618
Rwork	0.261
R-free	0.28660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hxm BTN2A1
RMSD bond length	0.008
RMSD bond angle	1.020
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.4 (11-DEC-2020))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.720	2.140
High resolution limit [Å]	2.100	2.100
Rpim	0.034	0.299
Number of reflections	77369	3834
<I/σ(I)>	13.78	2.84
Completeness [%]	100.0	100
Redundancy	13.4	13.8
CC(1/2)	0.998	0.725

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	20% PEG 3350, 0.2 M sodium malonate-malonic acid