8DFI
Crystal structure of moderately neutralizing / interfering human monoclonal antibody 42C11 Fab in complex with MSP1-19
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.560, 68.770, 63.730 |
| Unit cell angles | 90.00, 110.68, 90.00 |
Refinement procedure
| Resolution | 19.850 - 1.900 |
| R-factor | 0.1764 |
| Rwork | 0.175 |
| R-free | 0.20010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 1OB1 & 1AQK |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.698 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.850 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 37968 | 2198 |
| <I/σ(I)> | 18.85 | 4.86 |
| Completeness [%] | 94.5 | 74.5 |
| Redundancy | 3.45 | |
| CC(1/2) | 0.999 | 0.903 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.1 M HEPES, pH 7.5, 10 % (w/v) PEG 4000, 20% (w/v) Isopropanol |
