8DF2
The structure of the 'ALT' construct of the Amuc_1438 glycopeptidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-16 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54187 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.581, 146.094, 147.289 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.930 - 2.350 |
| R-factor | 0.2243 |
| Rwork | 0.222 |
| R-free | 0.26990 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.651 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rpim | 0.072 | 0.228 |
| Number of reflections | 78394 | 3818 |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 98.2 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M (NH4)2SO4, 20% (w/v) PEG3350 and 0.1M HEPES pH 7.5 |
