8DAE

Arabidopsis thaliana bifunctional dihydrofolate reductase-thymidylate synthase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 42 21 2
Unit cell lengths	80.717, 80.717, 181.757
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.381 - 2.000
Rwork	0.202
R-free	0.23730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3i3r
RMSD bond length	0.008
RMSD bond angle	1.502
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.381	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	41582	3010
<I/σ(I)>	18.2
Completeness [%]	100.0
Redundancy	26.3
CC(1/2)	1.000	0.370

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M ammonium sulfate, 0.1 M HEPES, pH 7.5, 35% w/v poly(acrylic acid sodium salt)2100