8D88

Structure of Y430F D-ornithine/D-lysine decarboxylase complex with D-lysine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	141.240, 50.490, 139.320
Unit cell angles	90.00, 117.26, 90.00

Refinement procedure

Resolution	41.280 - 1.410
R-factor	0.1752
Rwork	0.175
R-free	0.19460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6N2H.pdb
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.280	1.460
High resolution limit [Å]	1.410	1.410
Number of reflections	161759	12439
<I/σ(I)>	10.63
Completeness [%]	95.7
Redundancy	5.7
CC(1/2)	0.999	0.430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 H HEPES-K, pH 7.5, 0.2 M NaOAc, 20% PEG 4000