8D7M

Human Casein kinase 1 delta in complex with phosphorylated human PERIOD2 FASP peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03
Spacegroup name	C 1 2 1
Unit cell lengths	55.700, 135.970, 91.140
Unit cell angles	90.00, 94.33, 90.00

Refinement procedure

Resolution	46.155 - 2.250
R-factor	0.1835
Rwork	0.181
R-free	0.23330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pxo
RMSD bond length	0.009
RMSD bond angle	1.118
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.155	2.320
High resolution limit [Å]	2.250	2.250
Rmerge	0.152	1.270
Number of reflections	31319	2767
<I/σ(I)>	9.4	2.5
Completeness [%]	97.8
Redundancy	5.7
CC(1/2)	0.990	0.550

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.15 M Succinic Acid (5.5) 22% PEG 3350