8D73
Crystal Structure of EGFR LRTM with compound 7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2017-10-01 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.689, 49.448, 86.119 |
| Unit cell angles | 103.20, 101.57, 90.05 |
Refinement procedure
| Resolution | 29.790 - 2.170 |
| R-factor | 0.1957 |
| Rwork | 0.191 |
| R-free | 0.28530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4i22 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.407 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.800 | 2.290 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmeas | 0.130 | 0.660 |
| Number of reflections | 31680 | 4458 |
| <I/σ(I)> | 4.9 | |
| Completeness [%] | 91.4 | 88.5 |
| Redundancy | 1.7 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 285 | 0.10 M sodium acetate, pH 5.8, 0.30 M potassium acetate, 15% glycerol, 9% PEG 8000 |
