8D71

Human Ago2 bound to miR122(21nt)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-10-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	63.126, 107.735, 68.817
Unit cell angles	90.00, 106.89, 90.00

Refinement procedure

Resolution	39.200 - 2.500
R-factor	0.2253
Rwork	0.223
R-free	0.28020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ola
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.200	2.589
High resolution limit [Å]	2.500	2.500
Rmerge	0.019	0.143
Rmeas		0.202
Rpim		0.143
Number of reflections	30149	2990
<I/σ(I)>	23.81	4.81
Completeness [%]	98.7	99.27
Redundancy	2	2
CC(1/2)		0.956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1M Tris 8, 0.01 MgCl2, 0.1M Phenol,15% PEG 3350, 10% Isoproponal