8D5Q

TCR TG6 in complex with Ld-HF10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 41
Unit cell lengths	89.963, 89.963, 105.982
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.022 - 2.501
Rwork	0.169
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ld9 3to4
RMSD bond length	0.013
RMSD bond angle	1.892
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.000	2.540
High resolution limit [Å]	2.500	2.500
Rpim	0.030	0.290
Number of reflections	29168	2864
<I/σ(I)>	26.6
Completeness [%]	99.8
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	11 % w/v PEG 8000, 0.1 M MES 6.0, 0.24 M Ammonium sulfate