8D5K
The complex of Pre-mRNA-Processing Factor 19 (Prpf19) peptide KYLQVASHV Presented by H2-Kd
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-E |
| Synchrotron site | APS |
| Beamline | 21-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 115.370, 66.200, 56.790 |
| Unit cell angles | 90.00, 92.42, 90.00 |
Refinement procedure
| Resolution | 41.349 - 2.066 |
| Rwork | 0.203 |
| R-free | 0.23350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vgk |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.874 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0350) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.349 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.069 | 0.281 |
| Rmeas | 0.096 | 0.396 |
| Rpim | 0.067 | 0.278 |
| Number of reflections | 23784 | 8577 |
| <I/σ(I)> | 14.6 | |
| Completeness [%] | 90.4 | 97 |
| Redundancy | 2.3 | 1.9 |
| CC(1/2) | 0.993 | 0.899 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 10% w/v PEG8000, 200 mM magnesium acetate |
