8D5J

The complex of Pre-mRNA-Processing Factor 19 (Prpf19) neoantigen KYLQVASHV Presented by H2-Kd

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-E
Synchrotron site	APS
Beamline	21-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	115.440, 66.570, 56.960
Unit cell angles	90.00, 92.01, 90.00

Refinement procedure

Resolution	40.902 - 1.950
Rwork	0.186
R-free	0.22970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vgk
RMSD bond length	0.008
RMSD bond angle	1.701
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0350)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.902	40.870	2.000
High resolution limit [Å]	1.950	8.940	1.950
Rmerge	0.138	0.068	0.740
Rmeas	0.173	0.081	0.954
Rpim	0.103	0.044	0.594
Number of reflections	30593	2199	8577
<I/σ(I)>	15.9
Completeness [%]	97.1
Redundancy	4.7	6.3	4
CC(1/2)	0.991	0.995	0.682

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277.15	20% w/v PEG3000, 100 mM HEPES, 200 mM sodium acetate