8D5D

Structure of Y430F D-ornithine/D-lysine decarboxylase complex with D-arginine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	141.770, 49.540, 139.560
Unit cell angles	90.00, 115.93, 90.00

Refinement procedure

Resolution	48.860 - 1.540
R-factor	0.1615
Rwork	0.161
R-free	0.19270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6N2H.pdb
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.860	1.595
High resolution limit [Å]	1.540	1.540
Number of reflections	125192	9896
<I/σ(I)>	11.03	0.53
Completeness [%]	96.5
Redundancy	15.1
CC(1/2)	0.999	0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M HEPES, pH 7.5, 0.2 M NaOAc, 20% PEG 4000