8D47

fp.006 Fab in complex with SARS-CoV-2 Fusion Peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	90.017, 73.076, 90.117
Unit cell angles	90.00, 109.01, 90.00

Refinement procedure

Resolution	37.890 - 2.000
R-factor	0.1862
Rwork	0.184
R-free	0.23780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gxu 6fg1
RMSD bond length	0.007
RMSD bond angle	0.840
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.900	2.040
High resolution limit [Å]	2.000	2.000
Rmerge	0.117	0.996
Number of reflections	73148	4545
<I/σ(I)>	6.1	1.5
Completeness [%]	97.9	98.8
Redundancy	3	3
CC(1/2)	0.988	0.372

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.8	289	0.2 M Potassium phosphate monobasic, 20% w/v Polyethylene glycol 3350