8D3T

Crystal structure of GalS1 from Populus trichocarpas

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2018-09-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 1
Unit cell lengths	70.398, 83.422, 94.689
Unit cell angles	76.16, 69.67, 88.57

Refinement procedure

Resolution	80.840 - 2.370
R-factor	0.1976
Rwork	0.195
R-free	0.25210
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.572
Data reduction software	DIALS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.840	2.450
High resolution limit [Å]	2.370	2.370
Rmerge	0.103	1.036
Number of reflections	78131	7586
<I/σ(I)>	7.18
Completeness [%]	98.2
Redundancy	2.6
CC(1/2)	0.989	0.506

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Sodium citrate tribasic dihydrate pH 5.0, 10 % PEG 6,000