8D34

Crystal Structure of SARS CoV-2 NSP15 Endroribonuclease H250A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-26
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.54178
Spacegroup name	P 63
Unit cell lengths	152.525, 152.525, 109.644
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.130 - 2.910
R-factor	0.2471
Rwork	0.246
R-free	0.26260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vww
Data reduction software	HKL-2000 (v720)
Data scaling software	SCALEPACK (v2.3.15)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.960
High resolution limit [Å]	2.910	7.890	2.910
Rmerge	0.262	0.048	1.920
Rmeas	0.270	0.050	2.007
Rpim	0.068	0.013	0.578
Total number of observations	510947
Number of reflections	32039	1660	1565
<I/σ(I)>	4.3
Completeness [%]	100.0	99.9	100
Redundancy	15.9	14.7	11.9
CC(1/2)		0.999	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Calcium Acetate, 0.1 M Imidazole pH 8, 10% (w/v) PEG 8000