8D30
Crystal structure of the human COPB2 WD-domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.074, 134.062, 144.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.120 - 2.400 |
| R-factor | 0.221 |
| Rwork | 0.219 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nzr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.190 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 6.510 | 2.400 |
| Rmerge | 0.080 | 0.039 | 1.005 |
| Rmeas | 0.089 | 0.043 | 1.123 |
| Rpim | 0.037 | 0.018 | 0.486 |
| Total number of observations | 288205 | ||
| Number of reflections | 51291 | 2707 | 2439 |
| <I/σ(I)> | 8.9 | ||
| Completeness [%] | 99.0 | 96.2 | 97.1 |
| Redundancy | 5.6 | 5.4 | 4.8 |
| CC(1/2) | 0.997 | 0.496 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20%(w/v) PEG 3350, 0.05M Magnesium Formate |
