8D0Y

Crystal Structure of HIV-1 BG505 SOSIPv8 Trimer in Complex with CD4bs targeting antibody 21N13 and interface targeting antibody 35O22 at 4.7 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-06
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 3
Unit cell lengths	264.672, 264.672, 264.672
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.630 - 4.700
R-factor	0.2698
Rwork	0.268
R-free	0.29780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cez
RMSD bond length	0.004
RMSD bond angle	0.875
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.630	5.060	4.780
High resolution limit [Å]	4.700	4.960	4.700
Rmerge	0.240	2.880	2.900
Rmeas	0.240	3.031
Rpim	0.050	0.907	1.800
Number of reflections	32440	1624	1664
<I/σ(I)>	13.6
Completeness [%]	98.9	100	66.2
Redundancy	20.4	10.7	2.7
CC(1/2)		0.257	0.050

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M sodium acetate, pH5.5,1.75M Ammonium sulfate