8D0X
Human FUT9 bound to LNnT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-09 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 127.170, 127.170, 127.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.793 - 1.330 |
| R-factor | 0.1568 |
| Rwork | 0.156 |
| R-free | 0.17910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8d0o |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.904 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.800 | 1.360 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Rmeas | 0.134 | 2.070 |
| Number of reflections | 78129 | 7822 |
| <I/σ(I)> | 13.3 | 1.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 20.9 | 11.9 |
| CC(1/2) | 0.998 | 0.485 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.8 M Ammonium sulfate, 100 mM Tris pH 8.5 |
