8D0W

Human FUT9 bound to H-Type 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	I 21 3
Unit cell lengths	127.530, 127.530, 127.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.064 - 1.332
R-factor	0.1549
Rwork	0.154
R-free	0.17500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8d0o
RMSD bond length	0.005
RMSD bond angle	0.875
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.100	1.370
High resolution limit [Å]	1.330	1.330
Rmeas	0.091	2.090
Number of reflections	78345	7794
<I/σ(I)>	15.9	1.1
Completeness [%]	99.9	99.8
Redundancy	30.8	12.5
CC(1/2)	0.999	0.490

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	1.6 M Ammonium sulfate, 100 mM Tris pH 8.0