8D0P
Human FUT9, unliganded
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-09 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 127.350, 127.350, 127.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.090 |
| R-factor | 0.1423 |
| Rwork | 0.141 |
| R-free | 0.15990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8d0o |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.120 |
| High resolution limit [Å] | 1.090 | 1.090 |
| Rmeas | 0.087 | 1.700 |
| Number of reflections | 140941 | 13870 |
| <I/σ(I)> | 15.7 | |
| Completeness [%] | 99.9 | 98.9 |
| Redundancy | 17 | 12 |
| CC(1/2) | 0.990 | 0.510 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 1.6 M Ammonium sulfate, 100 mM Tris pH 8.0 |
