8CZH

Human BAK in complex with the dM2 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	102.130, 41.040, 47.020
Unit cell angles	90.00, 93.64, 90.00

Refinement procedure

Resolution	46.920 - 1.300
R-factor	0.1795
Rwork	0.178
R-free	0.20050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vx0
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.920	46.920	1.330
High resolution limit [Å]	1.300	5.810	1.300
Rmerge	0.040	0.025	1.714
Rmeas	0.043	0.027	1.854
Total number of observations	320911
Number of reflections	46424	571	3181
<I/σ(I)>	17.3	67.22	1.29
Completeness [%]	96.8	99	90.8
Redundancy	6.913	6.573	6.854
CC(1/2)	1.000	1.000	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	3.5 M sodium formate