8CYL

Ast89 P domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	291.15
Detector technology	PIXEL
Collection date	2017-11-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	74.590, 78.410, 100.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.415 - 1.888
R-factor	0.1644
Rwork	0.163
R-free	0.19490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4x1w
RMSD bond length	0.004
RMSD bond angle	0.770
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.425	42.425
High resolution limit [Å]	1.888	1.888
Number of reflections	47320	47320
<I/σ(I)>	9.19
Completeness [%]	98.2
Redundancy	1.36
CC(1/2)	0.903	0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.1 M citric acid (pH 4) and 30% PEG6000