8CXJ

The IgI3 domain of R28 protein from S. pyogenes bound to CEACAM1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1271
Spacegroup name	P 43
Unit cell lengths	76.962, 76.962, 334.849
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.960 - 3.050
R-factor	0.209
Rwork	0.207
R-free	0.23760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6v3p
RMSD bond length	0.002
RMSD bond angle	0.529
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (7.1.016)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.960	3.190
High resolution limit [Å]	3.050	3.050
Rmerge	0.076
Rpim	0.051
Number of reflections	36722	4528
<I/σ(I)>	16
Completeness [%]	99.9
Redundancy	6.2
CC(1/2)	0.999	0.828

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	1.45 M Ammonium sulfate 0.1M Na/K Phosphate pH 5.5