8CWT
Complex structure of WhiB3 and the SigmaAr4-RNAP Beta flap tip chimera in space group P43212
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 121.826, 121.826, 114.305 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.680 - 1.350 |
R-factor | 0.1528 |
Rwork | 0.152 |
R-free | 0.17390 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.673 |
Data reduction software | HKL-3000 (v1.0) |
Data scaling software | HKL-3000 (v1.0) |
Phasing software | AutoSol (v1.0) |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.370 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.082 | 3.020 |
Rmeas | 0.083 | 3.080 |
Rpim | 0.016 | 0.619 |
Number of reflections | 187184 | 18429 |
<I/σ(I)> | 48.2 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 25.3 | 24.7 |
CC(1/2) | 1.000 | 0.660 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 10-20 mM NiCl2, 0.8-1.0 M Li2SO4 |