8CVJ

Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-NH-tRNAphe, peptidyl P-site fMSEAC-NH-tRNAmet, and deacylated E-site tRNAphe at 2.40A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-08
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	210.470, 451.320, 627.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	109.890 - 2.400
R-factor	0.2488
Rwork	0.246
R-free	0.29480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhw
RMSD bond length	0.004
RMSD bond angle	0.872
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	109.890	109.890	2.460
High resolution limit [Å]	2.400	10.730	2.400
Rmerge	0.162	0.029	1.395
Rmeas	0.180	0.032	1.547
Total number of observations	11101606
Number of reflections	2249111	25975	161270
<I/σ(I)>	8.96	32.1	1
Completeness [%]	98.1	96.3	95.5
Redundancy	4.936	5.436	4.798
CC(1/2)	0.991	0.999	0.168

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME