8CTB

Human PRMT5:MEP50 structure with Fragment 3 and MTA Bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	I 2 2 2
Unit cell lengths	97.983, 137.232, 178.304
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.670 - 2.610
R-factor	0.2486
Rwork	0.246
R-free	0.28850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7uy1
RMSD bond length	0.002
RMSD bond angle	0.442
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.700	2.640
High resolution limit [Å]	2.610	2.610
Rmerge		0.600
Number of reflections	28091	427
<I/σ(I)>	9.4
Completeness [%]	76.1
Redundancy	2.7
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	10% PEG 8000, 100 mM Sodium Citrate 5.4, 100 mM Sodium Acetate