8CRI
Crystal structure of LplA1 in complex with lipoic acid (Listeria monocytogenes)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.870, 72.790, 93.050 |
| Unit cell angles | 90.00, 98.04, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.22487 |
| Rwork | 0.223 |
| R-free | 0.25145 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.155 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.055 | 0.660 |
| Number of reflections | 44270 | 5813 |
| <I/σ(I)> | 10.7 | 2.1 |
| Completeness [%] | 97.1 | 98.9 |
| Redundancy | 2.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 200 mM NH4Cl, 2.2 M (NH4)2SO4 |
