8CQX
Ribokinase from T.sp mutant A92G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-03 |
| Detector | DECTRIS EIGER R 1M |
| Wavelength(s) | 0.8856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.307, 155.608, 82.915 |
| Unit cell angles | 90.00, 96.37, 90.00 |
Refinement procedure
| Resolution | 29.520 - 2.270 |
| R-factor | 0.23322 |
| Rwork | 0.229 |
| R-free | 0.31148 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.670 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.390 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.133 | 0.340 |
| Rmeas | 0.158 | |
| Number of reflections | 51152 | 25537 |
| <I/σ(I)> | 3.886 | 2.03 |
| Completeness [%] | 99.4 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% peg3350, 0,45 M NaCl, 0,1 M citric acid pH 5.0, 5 mM MgCl2, 5 mM ADP |
