8CQP

Crystal structure of an 8-repeat consensus TPR superhelix with Calcium (low concentration)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97926
Spacegroup name	P 21 21 21
Unit cell lengths	66.448, 70.249, 102.619
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.970 - 1.700
R-factor	0.20311
Rwork	0.201
R-free	0.23897
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.545
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.250	1.790
High resolution limit [Å]	1.700	1.700
Rmeas	0.101	1.392
Rpim	0.041	0.571
Total number of observations	310018	44469
Number of reflections	53530	7724
<I/σ(I)>	7.9	1.4
Completeness [%]	99.9
Redundancy	5.8	5.8
CC(1/2)	0.995	0.477

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	292	CaCl2, 10 mM 100 mM NaOAc, pH 5.5 30% MPD