8CQM

Broad-range phospholipase C from Listeria monocytogenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.26
Spacegroup name	P 21 21 21
Unit cell lengths	34.909, 82.922, 140.815
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.460 - 2.000
R-factor	0.1758
Rwork	0.174
R-free	0.20890
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.906
Data reduction software	XDS (XDSAPP 2.0)
Data scaling software	XDS (XDSAPP 2.0)
Phasing software	AutoSol (1.19)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.940	2.120
High resolution limit [Å]	2.000	2.000
Rmeas	0.089	0.066
Number of reflections	53274	8615
<I/σ(I)>	27.07	4.26
Completeness [%]	99.6	99.5
Redundancy	14.5	13.5
CC(1/2)	1.000	0.934

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	294	PEG 8K Na-citrate isopropanol, trimethylamine-N-oxide, phoscholine