8CQK

pVHL:EloB:EloC in complex with (2S,4R)-1-((S)-2-(1-Fluorocyclopropane-1-carboxamido)-3,3-dimethylbutanoyl)-4-hydroxy-N-((S)-1-(2-methyl-4-(4-methylthiazol-5-yl)phenyl)ethyl)pyrrolidine-2-carboxamide (Compound 30)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.99999
Spacegroup name	P 41 21 2
Unit cell lengths	93.284, 93.284, 363.262
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.070 - 2.620
R-factor	0.2482
Rwork	0.246
R-free	0.28970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.059
Data reduction software	XDS (1.0.5)
Data scaling software	Aimless (0.7.8)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	181.630	2.760
High resolution limit [Å]	2.620	2.620
Rmerge	0.304	2.637
Rmeas	0.315	2.746
Rpim	0.082	0.759
Total number of observations	742511	91845
Number of reflections	49883	7147
<I/σ(I)>	7	1.1
Completeness [%]	100.0
Redundancy	14.9	12.9
CC(1/2)	0.995	0.428

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M sodium cacodylate, 15-20% polyethylene glycol 3350, 0.2 M magnesium acetate,10 mM DTT