8CQG

Crystal Structure of a Chimeric Alpha-Amylase from Pseudoalteromonas Haloplanktis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	68.879, 80.930, 128.481
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.480 - 1.740
R-factor	0.1779
Rwork	0.176
R-free	0.20510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.964
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.480	1.770
High resolution limit [Å]	1.740	1.740
Number of reflections	74537	3893
<I/σ(I)>	12.8
Completeness [%]	99.7
Redundancy	6.7
CC(1/2)	0.998	0.363

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	3 M NaCl, 0.1 M BisTris