8CQF

Crystal Structure of a Chimeric Alpha-Amylase from Pseudoalteromonas Haloplanktis Complexed with Rearranged Acarbose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-08
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.967700
Spacegroup name	P 21 21 21
Unit cell lengths	68.597, 81.071, 129.534
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.690 - 2.050
R-factor	0.1694
Rwork	0.169
R-free	0.19780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.204
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.690	2.123
High resolution limit [Å]	2.050	2.050
Number of reflections	84778	25013
<I/σ(I)>	6.5	1.1
Completeness [%]	96.7
Redundancy	2.9	2.9
CC(1/2)	0.993	0.471

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	3 M NaCl, 0.1 M BisTris