8CQ7

Crystal structure of phyllanthoside bound to the Candida albicans 80S ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	173
Detector technology	PIXEL
Collection date	2021-06-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	299.770, 294.170, 451.170
Unit cell angles	90.00, 100.05, 90.00

Refinement procedure

Resolution	228.180 - 3.200
R-factor	0.2466
Rwork	0.246
R-free	0.28110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.792
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19rc4_4035)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	228.180	228.180	3.250
High resolution limit [Å]	3.200	50.000	3.200
Rmerge	0.736	0.093	3.471
Rmeas	0.753	0.096	3.552
Number of reflections	1261990	296	57141
<I/σ(I)>	4.7
Completeness [%]	100.0
Redundancy	21.5
CC(1/2)	0.994	0.996	0.069

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277.15	PEG 20K, KSCN, Mg Acetate, K - acetate, Bis-Tris-Acetate, Glycerol, Spermidine, phyllanthoside