8CP7
Structure of the disulfide-locked substrate binding protein HiSiaP.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-09-30 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.826554 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 84.083, 89.451, 90.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.040 - 1.900 |
R-factor | 0.2127 |
Rwork | 0.210 |
R-free | 0.25840 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.033 |
Phasing software | PHENIX (dev_4788) |
Refinement software | PHENIX (dev_4788) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.040 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.029 | 0.613 |
Number of reflections | 26990 | 2437 |
<I/σ(I)> | 14.84 | |
Completeness [%] | 99.0 | |
Redundancy | 2 | |
CC(1/2) | 1.000 | 0.721 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.01 M Zinc chloride, 0.1 M Sodium acetate, pH 5.0, 20% w/v PEG 6000 |