8COB

Crystal structure of human PCNA in complex with ERCC6L2 PIP box peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-28
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.96600
Spacegroup name	P 21 21 21
Unit cell lengths	67.694, 109.082, 139.396
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.560 - 2.730
R-factor	0.1999
Rwork	0.197
R-free	0.25470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mlo
RMSD bond length	0.007
RMSD bond angle	1.139
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_3126)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.560	2.860
High resolution limit [Å]	2.730	2.730
Rmerge	0.118	0.531
Rmeas	0.133	0.599
Rpim	0.059	0.272
Number of reflections	28128	3678
<I/σ(I)>	10.5	3
Completeness [%]	99.9	99.9
Redundancy	5	4.8
CC(1/2)	0.995	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	25% (w/v) PEG3350, 100 mM BIS-TRIS pH 5.5