8CNT

Structure of the DEAH-box helicase Prp16 in complex with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976250
Spacegroup name	P 21 21 21
Unit cell lengths	55.130, 102.140, 106.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.970 - 1.900
R-factor	0.19527
Rwork	0.193
R-free	0.23819
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.486
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.970	50.000	2.000
High resolution limit [Å]	1.900	17.000	1.900
Rmerge	0.106	0.039	1.562
Rmeas	0.115	0.044	1.686
Number of reflections	91538	123	13012
<I/σ(I)>	11.78
Completeness [%]	99.9
Redundancy	7.07
CC(1/2)	0.999	0.998	0.503

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M MES pH 6.5 0.005 M Magnesiumchloride 20 % PEG 4000 (w/v)