8CNL

Lymphocytic choriomeningitis virus 3'-5' exonuclease domain of nucleoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976260
Spacegroup name	P 1 21 1
Unit cell lengths	95.076, 95.066, 129.130
Unit cell angles	90.00, 97.54, 90.00

Refinement procedure

Resolution	81.150 - 2.800
R-factor	0.2736
Rwork	0.273
R-free	0.27980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.368
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	94.254	94.254	4.470
High resolution limit [Å]	2.800	4.470	3.170
Number of reflections	310351	20129	36665
<I/σ(I)>	10.2	13.4	6.35
Completeness [%]	99.9
Redundancy	6.5
CC(1/2)	0.996	0.995	0.982

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	298	0.05M Sodium cacodylate pH 6.8, 0.01M Magnesium sulfate, 2M Ammonium sulfate