8CN3
hDLG1-PDZ2 in complex with a TAX1 peptide from HTLV-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.349, 53.569, 61.129 |
| Unit cell angles | 90.00, 93.33, 90.00 |
Refinement procedure
| Resolution | 40.260 - 2.710 |
| R-factor | 0.1901 |
| Rwork | 0.186 |
| R-free | 0.25910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.214 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.260 | 2.824 |
| High resolution limit [Å] | 2.706 | 2.706 |
| Rpim | 0.089 | 0.367 |
| Number of reflections | 4059 | 203 |
| <I/σ(I)> | 5.2 | |
| Completeness [%] | 90.1 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.990 | 0.803 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M Bis-Tris pH 5.5, 0.075M (NH4)2SO4, 24% PEG3350 |
