8CM0

Rhs2-CT endonuclease toxin in complex with cognate immunity protein RhsI2

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-09
Detector	DECTRIS PILATUS3 X 2M
Wavelength(s)	0.8731
Spacegroup name	P 21 21 21
Unit cell lengths	37.006, 81.931, 101.371
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.980 - 2.170
Rwork	0.195
R-free	0.25260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.477
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHASES
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.980	2.240
High resolution limit [Å]	2.170	2.170
Rmerge	0.157	1.511
Rpim	0.070	0.661
Number of reflections	17007	1408
<I/σ(I)>	8.7
Completeness [%]	100.0	100
Redundancy	6
CC(1/2)	0.993	0.542

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	Crystals were grown in a condition containing 0.2 uL of 6.5 mg/mL Rhs2CT-RhsI2 (in 20 mM MES & 20 mM imidazole pH 6.0, 150 mM NaCl, 1 mM MgCl2, 0.5 mM TCEP) and 0.2 uL of reservoir solution (0.2 M ammonium acetate, 0.1 M sodium acetate pH 5.0, 20% PEG4K).